Last edited by Vukazahn
Sunday, July 19, 2020 | History

6 edition of Culture of Human Stem Cells (Culture of Specialized Cells) found in the catalog.

Culture of Human Stem Cells (Culture of Specialized Cells)

by R. Ian Freshney

  • 86 Want to read
  • 24 Currently reading

Published by Wiley-Liss .
Written in English

    Subjects:
  • Developmental biology,
  • Genetics (non-medical),
  • Science,
  • Science/Mathematics,
  • Life Sciences - Cytology,
  • Research,
  • Research & Methodology,
  • Science / Biochemistry,
  • Life Sciences - Biochemistry,
  • Human cell culture,
  • Stem Cells

  • The Physical Object
    FormatHardcover
    Number of Pages343
    ID Numbers
    Open LibraryOL10278686M
    ISBN 100470052465
    ISBN 109780470052464

      Culture of Human Stem Cells Culture of Specialized Cells: : Freshney, R. Ian, Stacey, Glyn N., Auerbach, Jonathan M.: Books. Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis --Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov --Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen.

    Human embryonic stem cells (hESCs) hold great potential for the treatment of various degenerative diseases. Pluripotent hESCs have a great ability to undergo unlimited self-renewal in culture and to differentiate into all cell types in the body. The journey of hESC research is not that smooth, as it has faced several challenges which are limited to not only tumor formation and immunorejection. The process has been applied successfully in L hMSC expansion runs (3). hMSCs=human mesenchymal stem/stromal cells, PBS=phosphate buffer saline, FBS=fetal bovine serum. Viability, cell-surface characteristics, and functional attributes are commonly assessed following the detachment and harvest steps to ensure quality has been maintained.

    Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various by: 2. Leukemic stem cells (LSCs) are considered a major cause of relapse in acute myeloid leukemia (AML). Defining pathways that control LSC self-renewal is crucial for a better understanding of underlying mechanisms and for the development of targeted therapies. However, currently available culture condi .


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Culture of Human Stem Cells (Culture of Specialized Cells) by R. Ian Freshney Download PDF EPUB FB2

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series —each methods and protocols chapter is laid out.

Culture of Human Stem Cells includes a comprehensive list of suppliers for all equipment used in the protocols presented, with Web sites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer by:   Culture of Human Stem Cells book.

Read reviews from world’s largest community for readers. This book collects the most effective and cutting-edge methods Culture of Human Stem Cells book/5(3). Culture of Human Stem Cells, edited by R. Ian Freshney, Glyn N. Stacey, and Jonathan M. Auerbach, is a collection of established techniques presented in one volume that is designed to stand alone as a singular, definitive resource for anyone interested in the therapeutic potential of human stem by: 4.

This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series-each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data/5(3).

PDF | OnHeath D. Schmidt and others published Culture of Human Stem Cells (Culture of Specialized Cells) | Find, read and cite all the research you need on ResearchGate. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.

Key Features Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development. Book • 2nd Edition • Edited by: Growth Factors and the Serum-Free Culture of Human Pluripotent Stem Cells.

Book chapter Full text access. Chapter 29 - Growth Factors and the Serum-Free Culture of Human Pluripotent Stem Cells. Alice Pébay and Martin F. Pera. Pages The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture.

However, the successful culture and maintenance of human pluripotent stem cells (hPSCs) in an undifferentiated state requires additional considerations to ensure that cells maintain their key characteristics of self-renewal and.

Breast Cancer Stem Cells. Cancer theory suggests that the stem and progenitor cells of the mammary epithelium are the only cells long-lived enough to acquire the multiple genetic mutations required for malignant transformation.

17 While human mammary tumors have stem cell and non-stem cell components, only the stem cell fraction can generate new tumors upon transplantation into. This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells.

Twelve chapters - containing more than color illustrations - cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal.

Stem cell research is a rapidly expanding field with the potential to develop therapeutic agents to treat diseases as well as study disease development from early stages.

The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture. However, the successful culture and maintenance of human pluripotent stem cells (hPSCs) in an Cited by: 1.

Human pluripotent stem cells (hPSCs) are valuable tools for modeling human disease, as well as a source of differentiated cells for use in regenerative medicine and drug discovery. An essential prerequisite for using hPSCs in medicine is the assurance of. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America Includes new chapters devoted to recently developed methods, such as iPSC technology, written by.

Culture of human stem cells is used to expand the number of cells and differentiate the cells into various somatic cell types for transplantation. Stem cell culture is also used to harvest the molecules and exosomes that the stem cells release for the purposes of therapeutic development.

The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in volume will be the first to broadly cover multiple types of stem cell 4/5(1).

Quality control procedures for stem cell lines / Glyn N. Stacey and Jonathan M. Auerbach --Human embryonal stem cell lines: derivation and culture / Jessica A. Cooke and Stephen L. Minger --Techniques for neural differentiation of human EC and ES cells / Jamie P.

Jackson, Peter D. Tonge, and Peter W. Andrews --Cardiomyocyte differentiation in. Los Angeles, CA, USA – Scientists from the USC Stem Cell laboratories of Neil Segil and Justin Ichida are whispering the secrets of a simpler way to generate the sensory cells of the inner ear.

Their approach uses direct reprogramming to produce sensory cells known as “hair cells,” due to their hair-like protrusions that sense sound waves. This book details methods for derivation, culture, and differentiation of human stem cells obtained from both embryonic and adult sources.

The 13 chapters cover topics ranging from culture of the human germ cell lineage to multipotent stem cells in dental pulp to culturing mammary stem : $   Culture of human stem cells 1. CULTURE OF HUMAN STEM CELLS 2.

CULTURE OF HUMAN STEM CELLS Editors R. IAN FRESHNEY, PHD Centre for Oncology and Applied Pharmacology University of Glasgow Scotland, UK GLYN N. STACEY, PHD National Institute for Biological Standards and Control Potters Bar, Hertfordshire England, UK JONATHAN M.

Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells.

Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers.Protocol: Human Embryonic Stem Cell Culture Basement membranes are continuous sheets of specialized extracellular matrix that are found at the dermal-epidermal junction, at the base of all lumen-lining epithelia throughout the digestive, respiratory, reproductive, and urinary tracts, and that underlie parenchyma of endo-crine and exocrine glands.Embryonic stem cells (ESCs) are the cells of the inner cell mass of a blastocyst, formed prior to implantation in the uterus.

In human embryonic development the blastocyst stage is reached 4–5 days after fertilization, at which time it consists of 50– are pluripotent and give rise during development to all derivatives of the three germ layers: ectoderm, endoderm and mesoderm.